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Working with DNA in ERGO


How Do I View GenomeAlign Data?

You can find the GenomeAlign alignments available to you by going to the green "Data" drop down menu and selecting "GenomeAlign".

20081014_GenomeAlignDrop.jpg
Selecting GenomeAlign from the Data Drop Down Menu

After that, you can find the data you have access to in one of two ways.

20081014_GenomeAlignEntry.jpg
The GenomeAlign Menu Page

BLAST Menu Driven
You can follow the menu driven system that will allow you to set the BLAST score cutoff and length of BLAST hit that you want to use to compare your two genomes of interest. After you choose your BLAST thresholds and click the "Select BLAST cutoffs" button, you can select the Query organism from a drop down menu (the query organism is always the organism you are "BLASTing into" another organism) and then you can select the Target organism (the organism you are "BLASTing against"). The Target organism set will be smaller than the Query organism set since the data is pre-calculated and ERGO will filter the Target organism set based on the data available for the Query organism you selected.

Display All Available BLAST Runs
You can click the link "Display all available BLAST runs" (above the BLAST menu box) which will pull up a table of query and target organisms. Clicking on the "Show" link in the far right column next to any query/target pair will pull up the graphical display using the default BLAST settings (the default BLAST settings are the same as what you see when you first see the BLAST menu -- 1e-20 score cutoff and 200bp hit length cutoff.

20081014_GenomeAlignTable.jpg
Selecting Data from the List of Available BLAST Runs

Either of these entry points will take you to the graphical GenomeAlign view.

20081014_GenomeAlignView.jpg
GenomeAlign Viewer Page

You can mouse over any feature in either the query or target genome for more information about the feature and the alignment. For more information about the color coding and tool tip displays once you have the GenomeAlign page, please click on the blue "?" box in the "Alignment of BLAST hits from:" box at the top of the page to bring up the Help information.

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For general ERGO information and operating instructions, please check out our ERGO Manual.

We will be updating the manual on an ongoing basis, so be sure to check regularly for new information.

For technical support, please email us at:
ergo-support@integratedgenomics.com.

   
 
   
   
 
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